Use of an indirect haemagglutination test, for the detection of Clostridium perfringens type A enterotoxin

Autor: Marlene B. Serafim, Isildinha A. G. Colli, Lucila Costallat Ricci, Antonio F. Pestana de Castro
Jazyk: angličtina
Rok vydání: 1991
Předmět:
Zdroj: Memorias do Instituto Oswaldo Cruz, Vol 86, Iss 1, Pp 25-29 (1991)
Druh dokumentu: article
ISSN: 0074-0276
1678-8060
DOI: 10.1590/S0074-02761991000100005
Popis: An indirect haemagglutination (IH) test is described for the detection of Clostridium perfringens type A enterotoxin, produced by strains isolated from human cases of food poisoning and from contaminated food. Though no strict relationship could be observed between titers in the IH test and the time it took mice to die from the intravenous inoculation of mice (IIM), results of the supernatants examined by both methods demonstrated that the IH test was more sensitive than the ILM one. No unspecific reaction was obtanined int he IH wirh a negative control and the inhibitions of the IH and IIM tests by specific antiserum against C. perfringens enterotoxin showed that the IH test is very spcific. The IH assay is recommended for its sensitivity and easy performance by less-equipped laboratories, by these and other data.
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