Chitinolytic activity in viable spores of encephalitozoon species

Autor:	J Schottelius, F Hünger, Th Schüler, SC Gonçalves da Costa
Jazyk:	angličtina
Rok vydání:	2000
Předmět:	microspora cellulase chitinase activity inhibition Microbiology QR1-502 Infectious and parasitic diseases RC109-216
Zdroj:	Memorias do Instituto Oswaldo Cruz, Vol 95, Iss 5, Pp 701-705 (2000)
Druh dokumentu:	article
ISSN:	0074-0276 1678-8060
DOI:	10.1590/S0074-02762000000500015
Popis:	By employing 4-methylumbelliferyl-beta-D-NN',N"-triacetylchitotriose substrate in a semi quantitative assay, chitinolytic activity in viable spores of Encephalitozoon cuniculi and E. intestinalis was detected and dependence on reaction time, spore concentration, concentration of substrate and temperature were demonstrated. It was possible to block the chitinolytic activity by chitin hydrolysate. By incubation at 80°C for 10 min or at 55°C for 20 min the spores were loosing the chitinolytic activity. Incubation of the spores in trypsin reduced the chitinolytic activity. Cellulase activity could not be detected.
Databáze:	Directory of Open Access Journals
Externí odkaz:	https://doaj.org/article/806259bedc1041b6ae10f66623d0ea42 Zobrazit plný text záznamu View record in DOAJ