Anti-Müllerian hormone independently affect mtDNA copy number in human granulosa cells

Autor: Anom Bowolaksono, Ayu Mulia Sundari, Muhammad Fauzi, Mila Maidarti, Budi Wiweko, Kresna Mutia, Pritta Ameilia Iffanolida, Ririn Rahmala Febri, Astari Dwiranti, Hiroaki Funahashi
Jazyk: angličtina
Rok vydání: 2022
Předmět:
Zdroj: Journal of Ovarian Research, Vol 15, Iss 1, Pp 1-8 (2022)
Druh dokumentu: article
ISSN: 1757-2215
DOI: 10.1186/s13048-022-01047-4
Popis: Abstract Background: Recently, as a delayed childbearing trend is emerging in modern women’s adulthood, diminished reproductive potential due to age-related changes is more prevalent. Reduction in the abundance of mitochondrial DNA (mtDNA) copies and circulating anti-Müllerian hormone (AMH) have been separately reported with aging, contributing to the decrease in successful reproduction. However, there are limited reports on the impact of age on mtDNA and AMH in the same individual and whether mtDNA copy numbers are influenced by age and AMH. Methods: In the present study, we utilized a real-time quantitative PCR (RT-qPCR) to quantify the mtDNA copy number of granulosa cells obtained from 43 women undergoing an in vitro fertilization (IVF)/intracytoplasmic sperm injection (ICSI) program. Results: According to our analysis, a significant correlation was observed between age and mtDNA copy number (r = −0.54, P
Databáze: Directory of Open Access Journals
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