Utilization of Mn-Doped ZnSe/ZnS Core/Shell Quantum Dots for Rapid Detection of Escherichia coli O157:H7 and Methicillin-Resistant Staphylococcus aureus

Autor: Thi-Diem Bui, Quang-Liem Nguyen, Thi-Bich Luong, Van Thuan Le, Van-Dat Doan
Jazyk: angličtina
Rok vydání: 2020
Předmět:
Zdroj: Advances in Materials Science and Engineering, Vol 2020 (2020)
Druh dokumentu: article
ISSN: 1687-8434
1687-8442
DOI: 10.1155/2020/9718706
Popis: In this study, Mn-doped ZnSe/ZnS core/shell quantum dots (CSQDs) were synthesized in aqueous solution using polyethylene glycol as a surface stabilizer and successfully applied in the detection of Escherichia coli O157:H7 and methicillin-resistant Staphylococcus aureus (MRSA) for the first time. The CSQDs were conjugated with anti-E. coli antibody and anti-MRSA antibody via protein A supported by 1-ethyl-3-(-3-dimethylaminopropyl)carbodiimide hydrochloride for fluorescent labeling of the intact bacterial cells. The detection was performed for the bacterial strains cultivated in Luria-Bertani liquid medium. The obtained results indicate that E. coli O157:H7 and MRSA can be detected within 30 min at a high sensitivity of 101 CFU/mL. This labeling method based on the highly fluorescent CSQDs may have great potential for use in the food industry to check and prevent outbreaks of foodborne illness.
Databáze: Directory of Open Access Journals
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