Rose Bengal-Modified Upconverting Nanoparticles: Synthesis, Characterization, and Biological Evaluation

Autor: Mykhailo Nahorniak, Ognen Pop-Georgievski, Nadiia Velychkivska, Marcela Filipová, Eliška Rydvalová, Kristýna Gunár, Petr Matouš, Uliana Kostiv, Daniel Horák
Jazyk: angličtina
Rok vydání: 2022
Předmět:
Zdroj: Life, Vol 12, Iss 9, p 1383 (2022)
Druh dokumentu: article
ISSN: 12091383
2075-1729
DOI: 10.3390/life12091383
Popis: High-quality upconverting NaYF4:Yb3+,Er3+ nanoparticles (UCNPs; 26 nm in diameter) based on lanthanides were synthesized by a high-temperature coprecipitation method. The particles were modified by bisphosphonate-terminated poly(ethylene glycol) (PEG) and Rose Bengal (RB) photosensitizer. The particles were thoroughly characterized using transmission electron microscopy, dynamic light scattering, thermogravimetric analysis, FTIR, and X-ray photoelectron and upconversion luminescence spectroscopy in terms of morphology, hydrodynamic size, composition, and energy transfer to the photosensitizer. Moreover, the singlet oxygen generation from RB-containing UCNPs was investigated using 9,10-diphenylanthracene probe under 980 nm excitation. The cytotoxicity of UCNPs before and after conjugation with RB was evaluated on highly sensitive rat mesenchymal stem cells (rMSCs) and significant differences were found. Correspondingly, consi-derable variations in viability were revealed between the irradiated and non-irradiated rat glioma cell line (C6) exposed to RB-conjugated UCNPs. While the viability of rMSCs was not affected by the presence of UCNPs themselves, the cancer C6 cells were killed after the irradiation at 980 nm due to the reactive oxygen species (ROS) production, thus suggesting the potential of RB-conjugated PEG-modified UCNPs for applications in photodynamic therapy of cancer.
Databáze: Directory of Open Access Journals
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