Phenotypic and transcriptomic characterization of an ABA-sensitive mutant generated by microspore embryogenesis in barley

Autor: Yingbo Li, Guimei Guo, Hongwei Xu, Yingjie Zong, Shuwei Zhang, Linli Huang, Runhong Gao, Ruiju Lu, Longhua Zhou, Chenghong Liu
Jazyk: angličtina
Rok vydání: 2022
Předmět:
Zdroj: Journal of Plant Interactions, Vol 17, Iss 1, Pp 507-516 (2022)
Druh dokumentu: article
ISSN: 17429145
1742-9153
1742-9145
DOI: 10.1080/17429145.2022.2056250
Popis: Barley (Hordeum vulgare L.) has been recognized as an ideal model plant to study the mechanism of environmental adaptation for crop improvement owing to its wide adaption to abiotic stresses. Abscisic acid is an essential hormone involved in many abiotic stresses. Here, an ABA-sensitive barley mutant (abas1) was generated by microspore embryogenesis. On exogenous ABA treatment, the seedlings of abas1 presented less plant height, root length, shoot and root dry matter than the wild type, and more genes showedigher expressional intensity and up-regulation models by RNA-Seq profiling. The gene ontology analysis revealed that the regulation of DEGs may lead to the activation of the ROS pathway and consumption of more energy in abas1, specifically. Furthermore, the up-regulation of two candidate genes (P450 and NsLTP) in abas1 may contribute to the variation of ABA sensitivity. Our findings provide more valuable material and information toward the understanding of the mechanism of ABA response in barley.
Databáze: Directory of Open Access Journals
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