A simple, single-tube overlapping amplicon-targeted Illumina sequencing assay.

Autor:	Jason D Limberis, Alina Nalyvayko, Joel D Ernst, John Z Metcalfe
Jazyk:	angličtina
Rok vydání:	2023
Předmět:	Medicine Science
Zdroj:	PLoS ONE, Vol 18, Iss 9, p e0288687 (2023)
Druh dokumentu:	article
ISSN:	1932-6203
DOI:	10.1371/journal.pone.0288687
Popis:	Targeted amplicon sequencing to identify pathogens, resistance-conferring mutations, and strain types is an important tool in diagnosing and treating infections. However, due to the short read limitations of Illumina sequencing, many applications require the splitting of limited clinical samples between two reactions. Here, we outline hairpin Illumina single-tube sequencing PCR (hissPCR) which allows for the generation of overlapping amplicons containing Illumina indexes and adapters in a single tube, effectively extending the Illumina read length while maintaining reagent and sample input requirements.
Databáze:	Directory of Open Access Journals
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