Machine learning approaches for improving atomic force microscopy instrumentation and data analytics

Autor: Nabila Masud, Jaydeep Rade, Md. Hasibul Hasan Hasib, Adarsh Krishnamurthy, Anwesha Sarkar
Jazyk: angličtina
Rok vydání: 2024
Předmět:
Zdroj: Frontiers in Physics, Vol 12 (2024)
Druh dokumentu: article
ISSN: 2296-424X
DOI: 10.3389/fphy.2024.1347648
Popis: Atomic force microscopy (AFM) is a part of the scanning probe microscopy family. It provides a platform for high-resolution topographical imaging, surface analysis as well as nanomechanical property mapping for stiff and soft samples (live cells, proteins, and other biomolecules). AFM is also crucial for measuring single-molecule interaction forces and important parameters of binding dynamics for receptor-ligand interactions or protein-protein interactions on live cells. However, performing AFM measurements and the associated data analytics are tedious, laborious experimental procedures requiring specific skill sets and continuous user supervision. Significant progress has been made recently in artificial intelligence (AI) and deep learning (DL), extending into microscopy. In this review, we summarize how researchers have implemented machine learning approaches so far to improve the performance of atomic force microscopy (AFM), make AFM data analytics faster, and make data measurement procedures high-throughput. We also shed some light on the different application areas of AFM that have significantly benefited from applications of machine learning frameworks and discuss the scope and future possibilities of these crucial approaches.
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