Optimization of Platelet-Rich Plasma Preparation for Use in Clinical Practice
| Autor: | K. I. Burykin, N. V. Borovkova, M. S. Makarov, I. N. Ponomarev, M. V. Parshikov, N. V. Yarygin, A. M. Fain |
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| Jazyk: | ruština |
| Rok vydání: | 2023 |
| Předmět: | |
| Zdroj: | Неотложная медицинская помощь, Vol 12, Iss 2, Pp 268-273 (2023) |
| Druh dokumentu: | article |
| ISSN: | 2223-9022 2541-8017 |
| DOI: | 10.23934/2223-9022-2022-12-2-268-273 |
| Popis: | INTRODUCTION Development of effective biological products, based on human platelets, is very actual in regenerative medicine. The initial material for biological products’ obtaining is platelet-rich plasma (PRP), but the method of PRP isolation has not yet been standardized.AIM To assess the quality of platelets in PRP, harvested by different centrifugation modes.MATERIAL AND METHODS For platelet study, venous blood was harvested from volunteer donors. We used 3 methods for PRP-preparation, each methods included 2-stage centrifugation: 5 min 300g and 17 min 700g (Group 1); 10 min 300g and 10 min 700g (Group 2); 15 min 300g and 5 min 700g (Group 3). Platelets were examined using morphofunctional method based on vital cell staining.RESULTS In Group 1 and Group 2, the overall safety of biologically high-grade platelets in PRP was similar and estimated 55-60% of their total content in the blood. In Group 3 the safety of biologically high-grade platelets was only 30% (p |
| Databáze: | Directory of Open Access Journals |
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