Fate of (D-Ala2)-deltorphin-I-like immunoreactive neurons in 6-hydroxydopamine lesioned rat brain
Autor: | A Casini, A Pinna, I Tooyama, H Kimura, G Di Chiara, TG Renda |
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Jazyk: | angličtina |
Rok vydání: | 2009 |
Předmět: | |
Zdroj: | European Journal of Histochemistry, Vol 48, Iss 2 (2009) |
Druh dokumentu: | article |
ISSN: | 1121-760X 2038-8306 |
DOI: | 10.4081/879 |
Popis: | The use of a polyclonal antiserum specific to C-terminal tetrapeptide amide of (D-Ala2)deltorphin-I, a naturally occurring amphibian skin opioid peptide, has already demonstrated the presence of immunoreactive neurons in rat midbrain. Double immunostaining identified these neurons as a subpopulation of the mesencephalic dopaminergic neurons that were also tyrosine hydroxylase-immunopositive and calbindin- D28kD- negative, namely, the neurons predominantly affected in Parkinson disease. We followed the fate of these neurons after a monolateral injection of 6-hydroxy-dopamine into rat brain. Almost all the immunopositive neurons and their nigrostriatal, mesolimbic and mesocortical projections on the side ipsilateral to the lesion disappeared. Only a few scattered immunopositive neurons within the substantia nigra, pars compacta, and those of supramammillary nucleus remained unaffected. The consistent overlap of dopamine and this new molecule provides a further key to identifying the mammalian counterpart of these amphibian skin opioid peptides. |
Databáze: | Directory of Open Access Journals |
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