Abscisic acid and in vitro conservation of Manihot wild species

Autor: Jucieny Ferreira de Sá, Denise dos Santos Vila Verde, Marcus Dhilermando Hora de Souza, Jorge Eduardo dos Santos Melo, Karen Cristina Fialho dos Santos, Antonio da Silva Souza, Alfredo Augusto Cunha Alves, Carlos Alberto da Silva Ledo
Jazyk: angličtina
Rok vydání: 2022
Předmět:
Zdroj: Acta Scientiarum: Biological Sciences, Vol 44, Iss 1 (2022)
Druh dokumentu: article
ISSN: 1679-9283
1807-863X
DOI: 10.4025/actascibiolsci.v44i1.59549
Popis: Abscisic acid (ABA) is associated with bud dormancy, leaf abscission, and germplasm growth inhibition in in vitro conservation. We evaluated the effects of ABA in four wild Manihot accessions and one cassava accession (M. esculenta Crantz) to refine in vitro conservation methods for these species. The experiment was performed at the Laboratory for Tissue Culture from Embrapa, Cruz das Almas, Bahia State, Brazil. The statistical design was completely random in a 5 × 5 factorial scheme [(5 ABA dosages (0, 0.25, 0.50, 0.75, and 1 mg L-1) and 5 Manihot species (M. pseudoglaziovii, M. tristis, M. flabellifolia, M. chlorosticta, and M. esculenta)], with 15 replicates. Mini-cuttings of 1 cm were used, each inoculated in 10 mL of modified Murashige and Skoog medium, solidified with Phytagel® (2.4 g L-1) containing the respective ABA dosages. Tubes containing these mini-cuttings were placed in a germplasm conservation room with an irradiance of 30 µmol m-2 s-1, temperature of 22 ± 1°C, and photoperiod of 12 hours. Plant height (cm), the number of living and senescent leaves, shoots, and mini-cuttings (1 cm), and fresh and dry weights of shoots and roots (mg) were evaluated after 150 days. Growth reduction was prominent in M. pseudoglaziovii, M. tristis, and M. flabellifolia during the in vitro conservation period. In the present study, the addition of ABA did not promote the expected reduction in plant growth
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