Comparative seropositivity of in the serum of pregnant women with and without a history of abortion by serological and culture methods
| Autor: | Sahel Vafai, Mardin Maroofi Naghadehi, Fatemeh Keshavarzi |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2024 |
| Předmět: | |
| Zdroj: | SAGE Open Medicine, Vol 12 (2024) |
| Druh dokumentu: | article |
| ISSN: | 2050-3121 20503121 |
| DOI: | 10.1177/20503121241262189 |
| Popis: | Objective: Listeria monocytogenes is a major cause of miscarriage and postpartum infections in infant. Determining antibody levels against listeriolysin O can be valuable for diagnosing both invasive listeriosis and febrile gastroenteritis. However, serological methods that detect antibodies against incomplete forms of listeriolysin O can be more specific. The objective of this study was to identify ( Listeria monocytogenes ) in the serum of pregnant women using serological and culture methods. Methods: Clinical samples (120 cases) were collected from pregnant women with a gestational age of less than 20 weeks. Diagnosis of Listeria monocytogenes was conducted using culture methods to identify anti-Listeria antibodies. Statistical analysis of the results was conducted using IBM SPSS Statistics version 23.0 (New York, USA), Pearson’s Chi Square and fisher tests. Results: The number of positive samples by culture and ELISA was 24.16% (29) and 28.3% (34), respectively. Out of the 29 positive sample by the culture method, 10 individuals had no abortion history, 16 and 3 individuals had 1 and 2 abortions and no sample had 3 abortions. Maybe, the more abortions a person has had, the less likely they are to be infected. In the Enzyme Linked Immuno-Sorbent Assay (ELISA) method, 13 individuals tested positive for both IgG and IgM antibodies and 38 individuals tested negative. Additionally, among the positive individuals with 1, 2, and 3 miscarriages, 0, 17, and 3 people were positive for the IgG antibody and 0, 18, and 3 individuals were positive for the IgM antibody. The analysis results indicated that there was no significant relationship between culture and abortion history ( p = 0.316), IgG ELISA and history of miscarriage ( p = 0.672) and IgM ELISA and history of miscarriage ( p = 0.552). Conclusion: There was no significant relationship between infection with Listeria monocytogenes and abortion ( p ⩾ 0.05) in our samples. These results should be interpreted with caution due to the limitation of our small sample size. |
| Databáze: | Directory of Open Access Journals |
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