Caracterização química dos géis produzidos pelas bactérias diazotróficas Rhizobium tropici e Mesorhizobium sp.
Autor: | Nilson Kobori Monteiro, Gabriel Aranda-Selverio, Diego Tadeu Degli Exposti, Maria de Lourdes Corradi da Silva, Eliana Gertrudes Macedo Lemos, João Carlos Campanharo, Joana Léa Meira Silveira |
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Jazyk: | English<br />Spanish; Castilian<br />Portuguese |
Rok vydání: | 2012 |
Předmět: | |
Zdroj: | Química Nova, Vol 35, Iss 4, Pp 705-708 (2012) |
Druh dokumentu: | article |
ISSN: | 1678-7064 0100-4042 |
DOI: | 10.1590/S0100-40422012000400009 |
Popis: | The exopolysaccharides with characteristics of gel produced by Rhizobium tropici (EPS RT) and Mesorhizobium sp (EPS MR) are acidic heteropolysaccharide composed mainly of glucose and galactose in a molar ratio of 4:1 and 5:1 respectively, with traces of mannose (~ 1%). Chemical analysis showed the presence of uronic acid, pyruvate and acetyl-substituents in the structures of both polymers. Experiments of gel permeation chromatography and polyacrylamide gel electrophoresis showed that EPS RT and EPS MR are homogeneous molecules with low grade of polydispersity. The EPS were characterized using spectroscopic techniques of FT-IR, ¹H and 13C-NMR. |
Databáze: | Directory of Open Access Journals |
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