| Autor: |
Wei Xing, Ruihao Zhang, Qun Shao, Chunxiao Meng, Xiaodong Wang, Zuoxi Wei, Fengjie Sun, Chang Wang, Kai Cao, Bingkui Zhu, Zhengquan Gao |
| Jazyk: |
angličtina |
| Rok vydání: |
2021 |
| Předmět: |
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| Zdroj: |
Agronomy, Vol 11, Iss 5, p 961 (2021) |
| Druh dokumentu: |
article |
| ISSN: |
2073-4395 |
| DOI: |
10.3390/agronomy11050961 |
| Popis: |
Microalgae produce a variety of high-value products. Enhancing product contents in microalgal cells is one of the efficient ways to decrease production costs. Improved germplasm and heterotrophic cultivation may enhance microalgae biomass and lipid content. In this study, we investigated the effect of three types of laser irradiation and heterotrophic cultivation on lipid productivity, lipid content, and biomass of two Chlorella strains (i.e., FACHB 9 and FACHB 31). Results showed that the highest biomasses of 4.81 g/L (15.03-fold) and 4.66 g/L (7.32-fold) were obtained in the third generation of FACHB 9 and FACHB 31 induced by a neodymium-doped yttrium aluminum garnet (Nd:YAG) laser for 8 min and 12 min, respectively. The highest lipid contents were 525.6 mg/g (1.67-fold) dry weight (DW) and 780.0 mg/g DW (2.20-fold) in the third and the first generations of FACHB 9 and FACHB 31 induced by Nd:YAG for 8 min and by a helium–neon (He–Ne) laser for 4 min, respectively. The highest lipid productivities of 69.82 ± 3.29 mg/L/d (19.7-fold) and 30.71 ± 3.77 mg/L/d (3.1-fold) were obtained in FACHB 9 and FACHB 31 treated by a semiconductor (SC) laser for 4 min and by a He–Ne laser for 12 min, respectively. Our study suggested that laser mutagenesis is a potential method for screening economically important oleaginous microalgae strains. |
| Databáze: |
Directory of Open Access Journals |
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