| Autor: |
Zartasha Rehman, Mariam Naveed, Bushra Ijaz, Muhammad Musaddiq Shah, Imran Shahid, Mohammad Tarique Imam, Ziyad Saeed Almalki, Sidra Rehman |
| Jazyk: |
angličtina |
| Rok vydání: |
2023 |
| Předmět: |
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| Zdroj: |
Arabian Journal of Chemistry, Vol 16, Iss 12, Pp 105323- (2023) |
| Druh dokumentu: |
article |
| ISSN: |
1878-5352 |
| DOI: |
10.1016/j.arabjc.2023.105323 |
| Popis: |
Cancer nanotheranostic components are useful in monitoring drug delivery and potency against tumor cells. Current chemotherapeutic agents exhibit severe side effects and requires the urgency for discovery of potent therapeutic nano-drugs. So, this study focuses on evaluating the antitumor activity of chitosan nanoparticles encapsulating betanin (CSNPs-BET) against breast cancer (MDA-MB-231) cells. CSNPs-BET were prepared using the ionic gelation method and characterized for their size, polydispersity index (PDI), zeta potential, and surface morphology. Antitumor activity of CSNPs-BET and standard drug, doxorubicin (DOX), was evaluated using MTT, cell migration and gene expression assays. The entrapment efficiency of CSNPs-BET was in the approximate range of 88–91%. The sustained in vitro betanin release pattern was observed under different conditions. Chitosan nanoparticles encapsulated with and without betanin reduced the cell viability in MDA-MB-231 cells with IC50 values of 3.974 µg/mL and 7.994 µg/mL, respectively. Further, the treatment of MDA-MB-231 with CSNPs-BET with different concentrations significantly reduced the levels of PI3K (**P = 0.0016), Akt (**P = 0.0014) and mTOR (****P |
| Databáze: |
Directory of Open Access Journals |
| Externí odkaz: |
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