Production and Characterization of Chitooligosaccharides: Evaluation of Acute Toxicity, Healing, and Anti-Inflammatory Actions

Autor: Rafael Caetano Lisbôa Castro de Andrade, Nathália Kelly de Araújo, Manoela Torres-Rêgo, Allanny Alves Furtado, Alessandra Daniele-Silva, Weslley de Souza Paiva, Julia Maria de Medeiros Dantas, Nayara Sousa da Silva, Arnóbio Antônio da Silva-Júnior, Marcela Abbott Galvão Ururahy, Cristiane Fernandes de Assis, Leandro De Santis Ferreira, Hugo Alexandre Oliveira Rocha, Matheus de Freitas Fernandes-Pedrosa
Jazyk: angličtina
Rok vydání: 2021
Předmět:
Zdroj: International Journal of Molecular Sciences, Vol 22, Iss 19, p 10631 (2021)
Druh dokumentu: article
ISSN: 1422-0067
1661-6596
DOI: 10.3390/ijms221910631
Popis: The search for promising biomolecules such as chitooligosaccharides (COS) has increased due to the need for healing products that act efficiently, avoiding complications resulting from exacerbated inflammation. Therefore, this study aimed to produce COS in two stages of hydrolysis using chitosanases derived from Bacillus toyonensis. Additionally, this study aimed to structurally characterize the COS via mass spectrometry, to analyze their biocompatibility in acute toxicity models in vivo, to evaluate their healing action in a cell migration model in vitro, to analyze the anti-inflammatory activity in in vivo models of xylol-induced ear edema and zymosan-induced air pouch, and to assess the wound repair action in vivo. The structural characterization process pointed out the presence of hexamers. The in vitro and in vivo biocompatibility of COS was reaffirmed. The COS stimulated the fibroblast migration. In the in vivo inflammatory assays, COS showed an antiedematogenic response and significant reductions in leukocyte migration, cytokine release, and protein exudate. The COS healing effect in vivo was confirmed by the significant wound reduction after seven days of the experiment. These results indicated that the presence of hexamers influences the COS biological properties, which have potential uses in the pharmaceutical field due to their healing and anti-inflammatory action.
Databáze: Directory of Open Access Journals
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