Large Volume Electron Microscopy and Neural Microcircuit Analysis

Autor: Yoshiyuki Kubota, Jaerin Sohn, Yasuo Kawaguchi
Jazyk: angličtina
Rok vydání: 2018
Předmět:
Zdroj: Frontiers in Neural Circuits, Vol 12 (2018)
Druh dokumentu: article
ISSN: 1662-5110
DOI: 10.3389/fncir.2018.00098
Popis: One recent technical innovation in neuroscience is microcircuit analysis using three-dimensional reconstructions of neural elements with a large volume Electron microscopy (EM) data set. Large-scale data sets are acquired with newly-developed electron microscope systems such as automated tape-collecting ultramicrotomy (ATUM) with scanning EM (SEM), serial block-face EM (SBEM) and focused ion beam-SEM (FIB-SEM). Currently, projects are also underway to develop computer applications for the registration and segmentation of the serially-captured electron micrographs that are suitable for analyzing large volume EM data sets thoroughly and efficiently. The analysis of large volume data sets can bring innovative research results. These recently available techniques promote our understanding of the functional architecture of the brain.
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