β-arrestin2 Stimulates Interleukin-17 Production and Expression of CD4+ T Lymphocytes in a Murine Asthma Model
| Autor: | Yi Liu, Gu-yi Wang, Shao-kun Liu, Mu-yi Yang, Li-bing Ma, Keng Li, Su-bo Gong, Li Zhang, Ping Chen, Xu-dong Xiang |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2011 |
| Předmět: | |
| Zdroj: | Iranian Journal of Allergy, Asthma and Immunology, Vol 10, Iss 3 (2011) |
| Druh dokumentu: | article |
| ISSN: | 1735-1502 1735-5249 11772549 |
| Popis: | Allergic asthma is a complex and chronic inflammatory airway disease. Interleukin-17 is a pro-inflammatory cytokine which plays critical role in the pathogenesis of allergic asthma. It has been reported that β-arrestin2 regulated the development of allergic asthma at a proximal step in the inflammatory cascade. In this study, the influence of β-arrestin2 on Interleukin-17 production and expression of CD4+ T lymphocytes in a murine asthma model was investigated. Splenic CD4+ T lymphocytes from wild-type mice and those from a murine asthma model were purified. CD4+ T lymphocytes from a murine asthma model were transfected with siRNAs targeting the β-arrestin2 or were pretreated with the ERK1/2 inhibitor,PD98059. After stimulation, the protein expression of β-arrestin2、phosphorylated- ERK1/2 and IL-17 were detection by Western blot; the mRNA expression of IL-17 were detected by real-time PCR; the accumulation of IL-17 in supernatants were detected by ELISA. We found that β-arrestin2、phosphorylated-ERK1/2 and IL-17 expression in CD4+ T lymphocytes from a murine asthma model was increased compared with those from wild- type mice(p |
| Databáze: | Directory of Open Access Journals |
| Externí odkaz: |
|