Mammary core clock gene expression is impacted by photoperiod exposure during the dry period in goats

Autor: Theresa M. Casey, Karen Plaut, Mugagga Kalyesubula, Avi Shamay, Chris Sabastian, Yosi Wein, Enav Bar-Shira, Naama Reicher, Sameer. J. Mabjeesh
Jazyk: angličtina
Rok vydání: 2018
Předmět:
Zdroj: Journal of Applied Animal Research, Vol 46, Iss 1, Pp 1214-1219 (2018)
Druh dokumentu: article
ISSN: 0971-2119
0974-1844
09712119
DOI: 10.1080/09712119.2018.1486317
Popis: Short-day photoperiod (SDPP) during the dry period increases milk production compared to long-day photoperiod (LDPP) in goats. Photoperiod information is sent to the master clock in the suprachiasmatic nuclei (SCN), which send temporal information to peripheral clocks located in every tissue of the body. We hypothesized photoperiod effects on milk production are mediated in part by changes in mammary clocks. Our objective was to determine the effect of photoperiod manipulation during the dry period in goats on core clock genes expression in mammary gland. Multiparous goats (n = 6) were blocked at dry off into two treatments: LDPP and SDPP. Serial mammary biopsies were taken over a 24 h period during three weeks prepartum. Total RNA was isolated, and q-PCR analysis of the core clock genes CLOCK, ARNTL, PER1, CRY1, and CRY2 found exposure to LDPP significantly increased ARNTL (P
Databáze: Directory of Open Access Journals
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