| Autor: |
Karla F. Leavens, Catherine Osorio-Quintero, Elisabeth Ashlyne Yeuteuh, Francesca T. Perez-Profeta, Anna Ada Dattoli, Fabian L. Cardenas-Diaz, Deborah L. French, Paul Gadue |
| Jazyk: |
angličtina |
| Rok vydání: |
2024 |
| Předmět: |
|
| Zdroj: |
Stem Cell Research, Vol 81, Iss , Pp 103559- (2024) |
| Druh dokumentu: |
article |
| ISSN: |
1873-5061 |
| DOI: |
10.1016/j.scr.2024.103559 |
| Popis: |
Over the past decade, the use of human stem cell-derived β cells (SC-β cells) to model pancreatic β cell development, function and disease has become increasingly common. Though protocols are rapidly improving, current directed differentiation strategies do not yield a pure population of insulin-positive SC-β cells in vitro. Therefore, it is experimentally advantageous to have reporter lines that allow for live sorting of insulin-positive populations. To aid in these studies, we have knocked mNeonGreen fluorescent protein into the endogenous insulin locus of the commonly used H1 (WA01) human embryonic stem cell line. |
| Databáze: |
Directory of Open Access Journals |
| Externí odkaz: |
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