Combined microbiological approach to screening of producers of proteases with hemostasis system proteins activity among micromycetes

Autor: Alexander A. Osmolovskiy, Anna A. Lukianova, Elena S. Zvonareva, Valeriana G. Kreyer, Nina A. Baranova, Nikolay S. Egorov
Jazyk: angličtina
Rok vydání: 2018
Předmět:
Zdroj: Biotechnology Reports, Vol 19, Iss , Pp - (2018)
Druh dokumentu: article
ISSN: 2215-017X
DOI: 10.1016/j.btre.2018.e00265
Popis: A scheme for screening of micromycetes - producers of proteases with the activity of hemostasis system proteins, based on their enzymatic indices determination and the activity towards chromogenic peptide substrates for proteins of the hemostasis system was developed. Depending on the ability of proteases producers to cleave such substrates, an enzymatic reaction in conditions containing human plasma is suggested, which makes it possible to identify the potentiality of the target plasma hemostasis proenzymes activation.
Databáze: Directory of Open Access Journals