Optimization of Dengue Immunoassay by Label-Free Interferometric Optical Detection Method

Autor: María F. Laguna, Miguel Holgado, Francisco J. Sanza, Alvaro Lavín, Ana López, Rafael Casquel
Jazyk: angličtina
Rok vydání: 2014
Předmět:
Zdroj: Sensors, Vol 14, Iss 4, Pp 6695-6700 (2014)
Druh dokumentu: article
ISSN: 1424-8220
DOI: 10.3390/s140406695
Popis: In this communication we report a direct immunoassay for detecting dengue virus by means of a label-free interferometric optical detection method. We also demonstrate how we can optimize this sensing response by adding a blocking step able to significantly enhance the optical sensing response. The blocking reagent used for this optimization is a dry milk diluted in phosphate buffered saline. The recognition curve of dengue virus over the proposed surface sensor demonstrates the capacity of this method to be applied in Point of Care technology.
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