| Autor: |
Shaffifar, M., Tabarraei, A., Sajadian, A., Fotouhi, F, Ghaemi, A |
| Jazyk: |
angličtina |
| Rok vydání: |
2015 |
| Předmět: |
|
| Zdroj: |
Medical Laboratory Journal, Vol 9, Iss 1, Pp 9-16 (2015) |
| Druh dokumentu: |
article |
| ISSN: |
2538-4449 |
| Popis: |
Abstract Background and Objective: The M2 gene expressing the conserved protein in influenza virus can be used to make a single-dose vaccine with permanent immunity. Material and Methods: The mice were allocated to one case group immunized with pcDNA3-M2 and two control groups with pcDNA and PBS, in three dozes with interval of two weeks. Two weeks after the last injection, Cellular immunity was analyzed by MTT lymphocyte proliferation, interferon gamma (IFN-gamma) and interleukin 4 (IL-4) ratio assays. The remaining animals were challenged with PR8 virus. Results: The production rate of IFN8 and IL4 in pcDNA - M2 group was higher than that of control groups (P >0.0001). Given the results of lymphocyte proliferation, Stimulation index (SI) in vaccinated mice was significantly higher than that of control groups (P |
| Databáze: |
Directory of Open Access Journals |
| Externí odkaz: |
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