Lung recovery from DNA damage induced by graphene oxide is dependent on size, dose and inflammation profile

Autor: Luis Augusto Visani de Luna, Thomas Loret, Alexander Fordham, Atta Arshad, Matthew Drummond, Abbie Dodd, Neus Lozano, Kostas Kostarelos, Cyrill Bussy
Jazyk: angličtina
Rok vydání: 2022
Předmět:
Zdroj: Particle and Fibre Toxicology, Vol 19, Iss 1, Pp 1-21 (2022)
Druh dokumentu: article
ISSN: 1743-8977
DOI: 10.1186/s12989-022-00502-w
Popis: Abstract Background A key aspect of any new material safety assessment is the evaluation of their in vivo genotoxicity. Graphene oxide (GO) has been studied for many promising applications, but there are remaining concerns about its safety profile, especially after inhalation. Herein we tested whether GO lateral dimension, comparing micrometric (LGO) and nanometric (USGO) GO sheets, has a role in the formation of DNA double strand breaks in mouse lungs. We used spatial resolution and differential cell type analysis to measure DNA damages in both epithelial and immune cells, after either single or repeated exposure. Results GO induced DNA damages were size and dose dependent, in both exposure scenario. After single exposure to a high dose, both USGO and LGO induced significant DNA damage in the lung parenchyma, but only during the acute phase response (p
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