Comparison of the detection performance of two different one-step-combined test strips with fluorescent microspheres or colored microspheres as tracers for influenza A and B viruses

Autor: Qingjun Pan, Weiquan Wu, Shuzhen Liao, Sijie Wang, Chunfei Zhao, Chen Li, Ping Wu
Jazyk: angličtina
Rok vydání: 2019
Předmět:
Zdroj: Virology Journal, Vol 16, Iss 1, Pp 1-6 (2019)
Druh dokumentu: article
ISSN: 1743-422X
DOI: 10.1186/s12985-019-1190-0
Popis: Abstract Background Influenza A and B viruses mainly cause respiratory infectious disease. Till now, few tests are able to simultaneously detect both, especially in primary medical establishments. Methods This study was designed to compare the performance of two different one-step-combined test strips for the detection of influenza A and B: one strip with fluorescent microspheres for tracers (FMT); and the other strip with colored microspheres for tracers (CMT). To test the strips, cultures of influenza A, B, and other pathogenic viruses were used, in addition to 1085 clinical specimens from symptomatic patients with respiratory infections. Real-time RT-PCR was also considered as a reference method used to detect the different results of FMT and CTM. Results Detection thresholds for influenza A and B cultures using serial dilutions revealed that the sensitivity of FMT was higher than that of CMT (both P
Databáze: Directory of Open Access Journals
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