Improving indigenous Vietnamese Black Rabbit frozen sperm quality: the role of glycine and sperm selection methods
| Autor: | Tran Thi Thanh Khuong, Nguyen Lam Khanh Duy, Nguyen Thuy Hang, Phan Kim Ngoc, Duong Nguyen Duy Tuyen |
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| Jazyk: | angličtina |
| Rok vydání: | 2023 |
| Předmět: | |
| Zdroj: | World Rabbit Science, Vol 31, Iss 4, Pp 229-236 (2023) |
| Druh dokumentu: | article |
| ISSN: | 1257-5011 1989-8886 |
| DOI: | 10.4995/wrs.2023.19690 |
| Popis: | Rabbit sperm are known to undergo damage during both cryopreservation and thawing, leading to decreased viability, motility and membrane integrity. Glycine can protect sperm and reduce damage during freezing. Swim-up is a simple semen processing method for selecting good motile sperm. The study evaluated the effect of the swim-up method and glycine with different concentrations supplemented to the frozen medium. Three indigenous black rabbits were selected for semen collection by artificial vagina. Next, semen was selected by swim-up method and diluted with glycine-added frozen medium. The samples were then transferred to 0.5 mL straws, cooled to 15°C and 5°C, placed in liquid nitrogen vapour, and finally placed directly into liquid nitrogen (-196°C). The samples were thawed and evaluated for sperm quality. The results showed that the medium supplemented with 10mM glycine in combination with swim-up method for 30 min gave the best results and was significantly different from the remaining concentrations (P |
| Databáze: | Directory of Open Access Journals |
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