Latent pH-responsive ratiometric fluorescent cluster based on self-assembled photoactivated SNARF derivatives

Autor: Eiji Nakata, Yoshihiro Yukimachi, Yoshihiro Uto, Hitoshi Hori, Takashi Morii
Jazyk: angličtina
Rok vydání: 2016
Předmět:
Zdroj: Science and Technology of Advanced Materials, Vol 17, Iss 1, Pp 431-436 (2016)
Druh dokumentu: article
ISSN: 1468-6996
1878-5514
14686996
DOI: 10.1080/14686996.2016.1204888
Popis: We have developed a self-assembled fluorescent cluster comprising a seminaphthorhodafluor (SNARF) derivative protected by a photoremovable o-nitrobenzyl group. Prior to UV irradiation, a colorless and nonfluorescent cluster was spontaneously assembled in aqueous solution. After UV irradiation, the self-assembled cluster remained intact and showed a large enhancement in pH-responsive fluorescence. The unique pH responsive fluorescent cluster could be used as a dual-emissive ratiometric fluorescent pH probe not only in the test tube but also in HeLa cell cultures.
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