Identification of cryodamage on plasma membrane integrity in bull spermatozoa and its relationship with field fertility
| Autor: | Armando Quintero Moreno, Jorge Rubio Guillén, Decio González Villalobos, Juan Carlos Gutiérrez, Ninoska Madrid Bury, José Julián Garde López Brea |
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| Jazyk: | English<br />Spanish; Castilian |
| Rok vydání: | 2011 |
| Předmět: | |
| Zdroj: | Revista Científica, Vol 21, Iss 5 (2011) |
| Druh dokumentu: | article |
| ISSN: | 0798-2259 2521-9715 |
| Popis: | The aim of this study was to investigate the effect of cryopreservation on the membrane integrity (MI) in the bull sperm, and determine the relationship between intact cryopreserved sperm and field fertility (FF). The eosin nigrosin exclusion and the hypo-osmotic swelling test were combined in a single test (HOS-EN test) to identify the spermatozoa with four types of MI. After cryopreservation, there was a marked decline (21.3%) in the percentage of spermatozoa with head membrane intact/ tail membrane intact (Type IV MI), and a significant increase in those with head membrane damaged/ tail membrane damaged (Type I MI) (P |
| Databáze: | Directory of Open Access Journals |
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