Dynamic establishment and maintenance of the human intestinal B cell population and repertoire following transplantation in a pediatric-dominated cohort
| Autor: | Jianing Fu, Thomas Hsiao, Elizabeth Waffarn, Wenzhao Meng, Katherine D. Long, Kristjana Frangaj, Rebecca Jones, Alaka Gorur, Areen Shtewe, Muyang Li, Constanza Bay Muntnich, Kortney Rogers, Wenyu Jiao, Monica Velasco, Rei Matsumoto, Masaru Kubota, Steven Wells, Nichole Danzl, Shilpa Ravella, Alina Iuga, Elena-Rodica Vasilescu, Adam Griesemer, Joshua Weiner, Donna L. Farber, Eline T. Luning Prak, Mercedes Martinez, Tomoaki Kato, Uri Hershberg, Megan Sykes |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2024 |
| Předmět: | |
| Zdroj: | Frontiers in Immunology, Vol 15 (2024) |
| Druh dokumentu: | article |
| ISSN: | 1664-3224 91097401 |
| DOI: | 10.3389/fimmu.2024.1375486 |
| Popis: | IntroductionIt is unknown how intestinal B cell populations and B cell receptor (BCR) repertoires are established and maintained over time in humans. Following intestinal transplantation (ITx), surveillance ileal mucosal biopsies provide a unique opportunity to map the dynamic establishment of recipient gut lymphocyte populations in immunosuppressed conditions.MethodsUsing polychromatic flow cytometry that includes HLA allele group-specific antibodies distinguishing donor from recipient cells along with high throughput BCR sequencing, we tracked the establishment of recipient B cell populations and BCR repertoire in the allograft mucosa of ITx recipients.ResultsWe confirm the early presence of naïve donor B cells in the circulation (donor age range: 1-14 years, median: 3 years) and, for the first time, document the establishment of recipient B cell populations, including B resident memory cells, in the intestinal allograft mucosa (recipient age range at the time of transplant: 1-44 years, median: 3 years). Recipient B cell repopulation of the allograft was most rapid in infant ( |
| Databáze: | Directory of Open Access Journals |
| Externí odkaz: |
|