Changes in the Antioxidant Capacity and Iron-Binding Properties of Bovine Spermatozoa Following In Vitro Incubation with Ferrous or Ferric Iron
| Autor: | Eva Tvrdá, Anton Kováčik, Eva Tušimová, Peter Massányi, Norbert Lukáč |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2023 |
| Předmět: | |
| Zdroj: | Scientific Papers Animal Science and Biotechnologies, Vol 48, Iss 2, Pp 31-31 (2023) |
| Druh dokumentu: | article |
| ISSN: | 1841-9364 2344-4576 |
| Popis: | The aim of this study was to assess the impact of ferrous (Fe2+) or ferric (Fe3+) iron on the antioxidant capacity and the ability to bind iron of bovine spermatozoa at specific time intervals (0h, 2h, 8h, 16h and 24h) during an in vitro culture. 35 semen samples were collected from 7 adult breeding bulls and diluted in physiological saline solution supplemented with different concentrations (0, 1, 5, 10, 50, 100, 200, 500, 1000 μmol/L) of FeCl2 or FeCl3. Spermatozoa motility was assessed using the SpermVisionTM CASA (Computer aided sperm analysis) system. The ferric reducing ability of plasma (FRAP) assay was applied to study the antioxidant capacity of the samples, while the ability of the sample to bind excess iron was determined using the Total iron-binding capacity (TIBC) test. Both ferrous and ferric iron exhibited a dose- and time-dependent impact on the spermatozoa motility. Concentrations ≥50 µmol/L FeCl2 and ≥100 µmol/L FeCl3 led to a significant decrease of spermatozoa motion (P |
| Databáze: | Directory of Open Access Journals |
| Externí odkaz: |
|