| Autor: |
ZHANG Fu-yuan, WANG Lin-lin, ZHANG Miao, DONG Wen-wen, ZHANG Zhong-duo, LI Xin-jie, MA Xing-yu, DU Shu-kui, YUAN Hao-miao, GUAN Da-wei, ZHAO Rui |
| Jazyk: |
čínština |
| Rok vydání: |
2022 |
| Předmět: |
|
| Zdroj: |
Fayixue Zazhi, Vol 38, Iss 1, Pp 59-66 (2022) |
| Druh dokumentu: |
article |
| ISSN: |
1004-5619 |
| DOI: |
10.12116/j.issn.1004-5619.2021.410613 |
| Popis: |
ObjectiveThe metabolomics technique of LC-MS/MS combined with data analysis was used to detect changes and differences in metabolic profiles in the vitreous humor of early rat carcasses found in water, and to explore the feasibility of its use for early postmortem submersion interval （PMSI） estimation and the cause of death determination.MethodsThe experimental model was established in natural lake water with 100 SD rats were randomly divided into a drowning group （n=50） and a postmortem （CO2 suffocation） immediately submersion group （n=50）. Vitreous humor was extracted from 10 rats in each group at 0, 6, 12, 18 and 24 h postmortem for metabolomics analyses, of which 8 were used as the training set to build the model, and 2 were used as test set. PCA and PLS multivariate statistical analysis were performed to explore the differences in metabolic profiles among PMSI and causes of death in the training set samples. Then random forest （RF） algorithm was used to screen several biomarkers to establish a model.ResultsPCA and PLS analysis showed that the metabolic profiles had time regularity, but no differences were found among different causes of death. Thirteen small molecule biomarkers with good temporal correlation were selected by RF algorithm. A simple PMSI estimation model was constructed based on this indicator set, and the data of the test samples showed the mean absolute error （MAE） of the model was 0.847 h.ConclusionThe 13 metabolic markers screened in the vitreous humor of rat corpses in water had good correlations with the early PMSI. The simplified PMSI estimation model constructed by RF can be used to estimate the PMSI. Additionally, the metabolic profiles of vitreous humor cannot be used for early identification of cause of death in water carcasses. |
| Databáze: |
Directory of Open Access Journals |
| Externí odkaz: |
|
