Development and validation of a fast and simple RP-HPLC method for the determination of diosmin and hesperidin in combined tablet dosage form
| Autor: | Marjan Piponski, Tanja Bakovska Stoimenova, Marina Topkoska, Stefan Stefov, Magdalena Piponska, Gordana Trendovska Serafimovska |
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| Jazyk: | angličtina |
| Rok vydání: | 2018 |
| Předmět: | |
| Zdroj: | Macedonian Journal of Chemistry and Chemical Engineering, Vol 37, Iss 2, Pp 127-134 (2018) |
| Druh dokumentu: | article |
| ISSN: | 1857-5552 1857-5625 |
| DOI: | 10.20450/mjcce.2018.1448 |
| Popis: | A fast, simple, accurate and robust reversed phase HPLC method for the simultaneous determination of two flavonoids, hesperidin and diosmin, in combined tablets was developed and validated. This method uses a short C8 column with dimensions of 75 mm × 4 mm with 5 µm particles thermostated at 30 °C, and a mobile phase composed of formic acid (pH 4.1 and 0.05%, V/V) and methanol (58:42, V/V), delivered at a flow rate of 1.2 ml/min, with UV detector signal monitoring at 280 nm and an injection volume of 5 µl. These chromatographic conditions yielded chromatograms with symmetric peaks of hesperidin, eluting at a ~2 min retention time, and diosmin, at a ~4.5 min retention time, with a total run time cycle of 6 min. The method validation parameters confirmed excellent values for accuracy, linearity and reproducibility. This method is suitable for routine analysis in pharmaceutical and food quality control laboratories. |
| Databáze: | Directory of Open Access Journals |
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