| Autor: |
S L Fuller-Espie, L Goodfield, K Hill, K Grant, N DeRogatis |
| Jazyk: |
angličtina |
| Rok vydání: |
2008 |
| Předmět: |
|
| Zdroj: |
Invertebrate Survival Journal, Vol 5, Iss 2 (2008) |
| Druh dokumentu: |
article |
| ISSN: |
1824-307X |
| Popis: |
This study was aimed at determining the influence of human proinflammatory cytokines on innate immune responses in the earthworm Eisenia hortensis. Preincubation of earthworm coelomocytes in vitro with either interleukin-1 beta (IL-1 beta), granulocyte-macrophage colony stimulating factor (GMCSF), interleukin-2 (IL-2), or tumor necrosis factor-alpha (TNF-alpha) followed by subsequent bacterial challenge was carried out to investigate whether human proinflammatory cytokines would induce a state of enhanced responsiveness in phagocytic cells derived from the coelomic cavity of E. hortensis. The effect on phagocytosis by large coelomocytes (hyaline amebocytes) was evaluated using flow cytometry where the uptake of Escherichia coli expressing green fluorescence protein in the presence or absence of pretreatment with proinflammatory cytokines was measured. Our results show that proinflammatory cytokines enhanced phagocytosis to a statistically significant (p ≤ 0.05) degree in 10- 18 % of earthworms tested for IL-1 beta, 20 % for GM-CSF, 20-27 % for IL-2, and 27-30 % for TNFalpha, depending on the cytokine concentration used. Our results favor the suggestion that receptorcoding genes have been conserved through evolution between vertebrates and invertebrates. |
| Databáze: |
Directory of Open Access Journals |
| Externí odkaz: |
|
