| Autor: |
Hui Wang, Zheng Liu, Wenxiu Zhang, Ziao Yuan, Hongyi Yuan, Xueting Liu, Chunwen Yang, Weijun Guan |
| Jazyk: |
angličtina |
| Předmět: |
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| Zdroj: |
Biological Research, Vol 49, Iss 0, Pp 1-14 |
| Druh dokumentu: |
article |
| ISSN: |
0716-9760 |
| DOI: |
10.1186/s40659-016-0103-6 |
| Popis: |
BACKGROUND: Heavy metals can cause great harm to Siberian tigers in the natural environment. Cadmium (Cd2+) is an environmental contaminant that affects multiple cellular processes, including cell proliferation, differentiation, and survival. It has been shown to induce apoptosis in a variety of cell types and tissues. RESULTS: We investigated the apoptotic effects of Cd2+ on Siberian tiger fibroblasts in vitro. Our research revealed the typical signs of apoptosis after Cd²+ exposure. Apoptosis was dose- (0-4.8 μΜ) and duration-dependent (12-48 h), and proliferation was strongly inhibited. Cd²+ increased the activity of caspase-3, -8, and -9 and disrupted calcium homeostasis by causing oxidative stress and mitochondrial dysfunction. It also increased K+ efflux and altered the mRNA levels of Bax, Bcl-2, caspase-3, caspase-8, Fas, and p53. CONCLUSIONS: Our results suggest that Cd2+ triggers the apoptosis of Siberian tiger fibroblasts by disturbing intracellular homeostasis. These results will aid in our understanding of the effects of Cd2+ on Siberian tigers and in developing interventions to treat and prevent cadmium poisoning. |
| Databáze: |
Directory of Open Access Journals |
| Externí odkaz: |
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