PENGARUH EKSTRAK DIKLOROMETAN JAHE (Zingiber officinale Roscoe) TERHADAP PENGIKATAN TOKSIN KOLERA B-subunit CONJUGASI (FITC) PADA RECEPTOR SEL HIBRIDOMA LV DAN CACO-2 [Effect of Ginger (Zingiber officinale Roscoe) Dichloromethane Extract on Inhibition of Cholera Toxin B-subunit Conjugated FITC Binding to Receptor on LV Hibridome and Caco-2 Cells]
| Autor: | Radiati, L.E 1), Nabet, P , Franck, P 2), Nabet B 2), Capiaumont, J 2), Fardiaz, D 3), Zakaria, R.F 3), I. Sudirman 4), Hariyadi, R.D 3) |
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| Jazyk: | English<br />Indonesian |
| Rok vydání: | 2003 |
| Předmět: | |
| Zdroj: | Jurnal Teknologi dan Industri Pangan, Vol 14, Iss 1, Pp 59-67 (2003) |
| Druh dokumentu: | article |
| ISSN: | 1979-7788 2087-751X |
| Popis: | Cholera toxin is main factor that responsibility of watery diarrhea. The objectives of this investigation was to study the effect of ginger extract (GDE) in blocking cholera toxin binding to receptors of LV hybridome and Caco-2 cells.Analysis of toxin binding by flow cytometry of 0-5 g/ml of B-FITC conjugated cholera toxin to 105 cells/ml of LV hybridome and Caco-2 cells in RPMI, at 4C for one hour showed that specific interaction of B-FITC by 44,44 3,49 percent to LV hybridome and by 94,58 1,83 percent to Caco-2 cells A total of 105 cells/ml of hybridome and Caco-2 cells were incubated with 0-5 g/ml toxin B-FITC and 25 or 50 g/ml GDE in RPMI, at 4C for one hour showed that the addition of GDE inhibited the toxin binding. The binding inhibition respectively were 4.76-15.66 and 12.55-24.60 percent to Caco-2 cells, and 3.55-17.95 and 3.58-27.83 percent to hybridome cells. The inhibition on the toxin binding may be due to modification of the receptor by GDE or interaction of GDE with the toxin. |
| Databáze: | Directory of Open Access Journals |
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