Molecular characterization of the NRAMP1 gene in buffalo
| Autor: | Bianca Moioli, Corrado Pacelli, Federica Signorelli, Gennaro Catillo, Alessandra Crisà, Maria Carmela Scatà, Francesco Napolitano, Giovanna De Matteis |
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| Jazyk: | angličtina |
| Rok vydání: | 2010 |
| Předmět: | |
| Zdroj: | Italian Journal of Animal Science, Vol 8, Iss 2s, Pp 72-74 (2010) |
| Druh dokumentu: | article |
| ISSN: | 1594-4077 1828-051X |
| DOI: | 10.4081/ijas.2009.s2.72 |
| Popis: | NRAMP1 (natural-resistance-associated macrophage protein) gene influences the initial phase of bacterial cellular infections, regulating macrophage activation. Recent literature on buffalo has attempted to associate the genotypes at the polymorphic microsatellite, that is located in the 3'-UTR of the gene, with either susceptibility to brucellosis or improved macrophage function. However, contradictory results were reported. In the present work, we have sequenced the whole coding region, as well as part of the introns and UTRs, of the NRAMP1 gene in 49 Mediterranean buffaloes, including both serologically positive and negative animals to Brucella abortus test. We have detected 12 mutations. Nineteen haplotypes were built from the detected variant alleles, so demonstrating the high variability of this gene in buffalo, but no significant differences in haplotype frequencies were found between serologically positive/negative animals. |
| Databáze: | Directory of Open Access Journals |
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