Amino acid supplementation affects imprinted gene transcription patterns in parthenogenetic porcine blastocysts.

Autor: Chi-Hun Park, Young-Hee Jeong, Yeun-Ik Jeong, Jeong-Woo Kwon, Taeyoung Shin, Sang-Hwan Hyun, Eui-Bae Jeung, Nam-Hyung Kim, Sang-Kyo Seo, Chang-Kyu Lee, Woo-Suk Hwang
Jazyk: angličtina
Rok vydání: 2014
Předmět:
Zdroj: PLoS ONE, Vol 9, Iss 9, p e106549 (2014)
Druh dokumentu: article
ISSN: 1932-6203
DOI: 10.1371/journal.pone.0106549
Popis: To determine whether exogenous amino acids affect gene transcription patterns in parthenogenetic porcine embryos, we investigated the effects of amino acid mixtures in culture medium. Parthenogenetic embryos were cultured in PZM3 medium under four experimental conditions: 1) control (no amino acids except L-glutamine and taurine); 2) nonessential amino acids (NEAA); 3) essential amino acids (EAA); and 4) NEAA and EAA. The rate of development of embryos to the four-cell stage was not affected by treatment. However, fewer (P
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