The use of lysozyme to prepare biologically active chitooligomers

Autor: Anna Zimoch-Korzycka, Christian Gardrat, Alain Castellan, Véronique Coma, Andrzej Jarmoluk
Jazyk: English<br />Portuguese
Rok vydání: 2015
Předmět:
Zdroj: Polímeros, Vol 25, Iss 1, Pp 35-41 (2015)
Druh dokumentu: article
ISSN: 1678-5169
0104-1428
DOI: 10.1590/0104-1428.1630
Popis: Two types of crustacean commercial chitosans (CS1, CS2) were dissolved in lactic acid solutions, hydrolysed by lysozyme and finally fractioned by methanol solutions into two parts containing chito-oligomers (CS-O1, CS-O2). The antioxidant power and antimicrobial properties of both fractions were studied and compared with non-hydrolysed CS1 and CS2. The antioxidant properties were determined by the ferric ion reducing antioxidant power (FRAP) method while the bioactive properties were evaluated against a strain of Listeria monocytogenes. CS-O obtained from the solid fraction of the chito-oligomers solid fractions treated with 90% methanol showed the highest reducing power. Microbiological tests showed that CS-O exhibit higher antilisterial activity than CS.
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