| Autor: |
Cynthia M. Galicia-Medina, Matías Vázquez-Piñón, Gibran S. Alemán-Nava, Roberto C. Gallo-Villanueva, Sergio O. Martínez-Chapa, Marc J. Madou, Sergio Camacho-León, Jonathan S. García-Pérez, Diego A. Esquivel-Hernández, Roberto Parra-Saldívar, Víctor H. Pérez-González |
| Jazyk: |
angličtina |
| Rok vydání: |
2021 |
| Předmět: |
|
| Zdroj: |
Micromachines, Vol 12, Iss 9, p 1023 (2021) |
| Druh dokumentu: |
article |
| ISSN: |
2072-666X |
| DOI: |
10.3390/mi12091023 |
| Popis: |
In this study, we carried out a heterogeneous cytoplasmic lipid content screening of Neochloris oleoabundans microalgae by dielectrophoresis (DEP), using castellated glassy carbon microelectrodes in a PDMS microchannel. For this purpose, microalgae were cultured in nitrogen-replete (N+) and nitrogen-deplete (N−) suspensions to promote low and high cytoplasmic lipid production in cells, respectively. Experiments were carried out over a wide frequency window (100 kHz–30 MHz) at a fixed amplitude of 7 VPP. The results showed a statistically significant difference between the dielectrophoretic behavior of N+ and N− cells at low frequencies (100–800 kHz), whereas a weak response was observed for mid- and high frequencies (1–30 MHz). Additionally, a finite element analysis using a 3D model was conducted to determine the dielectrophoretic trapping zones across the electrode gaps. These results suggest that low-cost glassy carbon is a reliable material for microalgae classification—between low and high cytoplasmic lipid content—through DEP, providing a fast and straightforward mechanism. |
| Databáze: |
Directory of Open Access Journals |
| Externí odkaz: |
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