Targeted mutagenesis of StISAC stabilizes the production of anthocyanins in potato cell culture

Autor: Vincenzo D'Amelia, Annalisa Staiti, Fabio D'Orso, Maria Maisto, Vincenzo Piccolo, Riccardo Aversano, Domenico Carputo
Jazyk: angličtina
Rok vydání: 2022
Předmět:
Zdroj: Plant Direct, Vol 6, Iss 8, Pp n/a-n/a (2022)
Druh dokumentu: article
ISSN: 2475-4455
DOI: 10.1002/pld3.433
Popis: Abstract To increase the production of decorated anthocyanins in potato cell cultures, we knocked out a novel potato gene, named Inducer Silencing of Anthocyanins in Cell culture (StISAC), using CRISPR‐Cas9 editing. Our results provided evidence that mutant cell lines doubled the accumulation level of anthocyanins biosynthesized. Moreover, the production of these important pigments was stabilized over time. Our study overcame important challenges in the efficient biotechnological production of these valuable pigments and reported the function of a novel anthocyanin biosynthesis repressor gene.
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