Strategies and Applications of Antigen-Binding Fragment (Fab) Production in Escherichia coli
| Autor: | Hui Chen, Jun-Sheng Chen, Pameila Paerhati, Tanja Jakos, Si-Yi Bai, Jian-Wei Zhu, Yun-Sheng Yuan |
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| Jazyk: | angličtina |
| Rok vydání: | 2021 |
| Předmět: | |
| Zdroj: | Pharmaceutical Fronts, Vol 03, Iss 02, Pp e39-e49 (2021) |
| Druh dokumentu: | article |
| ISSN: | 2628-5088 2628-5096 |
| DOI: | 10.1055/s-0041-1735145 |
| Popis: | With the advancement of genetic engineering, monoclonal antibodies (mAbs) have made far-reaching progress in the treatment of various human diseases. However, due to the high cost of production, the increasing demands for antibody-based therapies have not been fully met. Currently, mAb-derived alternatives, such as antigen-binding fragments (Fab), single-chain variable fragments, bispecifics, nanobodies, and conjugated mAbs have emerged as promising new therapeutic modalities. They can be readily prepared in bacterial systems with well-established fermentation technology and ease of manipulation, leading to the reduction of overall cost. This review aims to shed light on the strategies to improve the expression, purification, and yield of Fab fragments in Escherichia coli expression systems, as well as current advances in the applications of Fab fragments. |
| Databáze: | Directory of Open Access Journals |
| Externí odkaz: |
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