Diversity and antibiotic resistance patterns of enterobacteria isolated from seafood in Thailand

Autor: Neelawan Pongsilp, Pongrawee Nimnoi
Jazyk: English<br />Spanish; Castilian
Rok vydání: 2018
Předmět:
Zdroj: CyTA - Journal of Food, Vol 16, Iss 1, Pp 793-800 (2018)
Druh dokumentu: article
ISSN: 1947-6337
1947-6345
19476337
DOI: 10.1080/19476337.2018.1479453
Popis: Contamination with enterobacteria was detectable in 89% of seafood samples from three central seafood markets in Thailand. The average numbers obtained from the same type of seafood were between 1.3 ± 0.9 and 4.5 ± 1.3 log CFU/g per sample. Eighty-one strains and 16 species were distinguished based on ERIC-PCR patterns and TP-RAPD patterns, respectively. The highest prevalence (90% of strains) was resistant to penicillin G whereas none was resistant to gentamycin. In addition, 63% exhibited multidrug resistance. The 16S rDNA sequences of a representative strain from each species exhibited 99% identity to either one of six genera including Citrobacter, Enterobacter, Klebsiella, Providencia, Serratia, and Yersinia. Three β-lactamase genes including blaTEM, ampC, and shv were detected at the frequencies of 43%, 27%, and 24%, respectively. The representative strains possessing β-lactamase genes exhibited β-lactamase activity ranging from 1.96 ± 0.88 to 11.3 ± 0.37 μmol of hydrolyzed nitrocefin/min/mg protein.
Databáze: Directory of Open Access Journals
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