| Autor: |
Mauricio Suarez, Sergios Charntikov, Y. Wendy Huynh, Scott T. Barrett, Rick A. Bevins, Ken T. Wakabayashi |
| Jazyk: |
angličtina |
| Rok vydání: |
2024 |
| Předmět: |
|
| Zdroj: |
MethodsX, Vol 12, Iss , Pp 102675- (2024) |
| Druh dokumentu: |
article |
| ISSN: |
2215-0161 |
| DOI: |
10.1016/j.mex.2024.102675 |
| Popis: |
Intravenous self-administration in rats is used widely to study the reinforcing effects of drugs and serves as the gold standard for assessing their use and misuse potential. One challenge that researchers often encounter when scaling up experiments is balancing the cost, time investment to construct, and robustness of each implanted catheter. These catheters include multiple components such as surgical meshing and a variety of entry ports designed to facilitate the connection of the rat to a catheter port tethering system. Other considerations include maintaining the catheters free of blockage during the extent of the drug self-administration experiment. These large-scale studies provide ample opportunity for the catheter system to fail. The failure and replacement of commercially purchased catheters leads to ballooning expenses, and the failure of in-lab manufactured catheters requires the manufacture of reserves, also increasing costs, as these handmade products are inherently more variable. We have developed a catheter system that combines a commercially available implantable back-mounted entry connector system with inexpensive medical items such as surgical mesh, sutures, and an air-tight back flow prevention system to bolster the overall success of self-administration experiments. • Method to bolster commercially available jugular catheter components for long-lasting self-administration experiments. • Reduces the overall cost per unit of self-administration experiments. • Easily assembled by laboratory students and staff. |
| Databáze: |
Directory of Open Access Journals |
| Externí odkaz: |
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