Noninvasive genetic sampling of endangered muriqui (Primates, Atelidae): efficiency of fecal DNA extraction
| Autor: | Paulo B. Chaves, Marcela F. Paes, Sérgio L. Mendes, Karen B. Strier, Iúri D. Louro, Valéria Fagundes |
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| Jazyk: | angličtina |
| Rok vydání: | 2006 |
| Předmět: | |
| Zdroj: | Genetics and Molecular Biology, Vol 29, Iss 4, Pp 750-754 (2006) |
| Druh dokumentu: | article |
| ISSN: | 1415-4757 1678-4685 48026468 |
| DOI: | 10.1590/S1415-47572006000400028 |
| Popis: | The muriqui (Brachyteles) is one of the most endangered primates in the world, however little is known about the viability of the remaining populations. We evaluated the technique of extracting DNA from wild muriqui feces for PCR applications. In order to determine the effect of the DNA in subsequent amplifications, we analyzed five different extracts. The importance of the recommended BSA and the HotStarTaq DNA polymerase was tested. The minimal conditions to successfully amplify highly degraded fecal DNA were determined, showing that the recommended reagents are not required. We envision that this method may be useful in further conservation management studies. |
| Databáze: | Directory of Open Access Journals |
| Externí odkaz: |
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