A rapid and simple bead-bashing-based method for genomic DNA extraction from mammalian tissue
| Autor: | Shan Wei, Brynn Levy, Nataly Hoffman, Claudia Cujar, Reunet Rodney-Sandy, Ronald Wapner, Mary D'Alton, Zev Williams |
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| Jazyk: | angličtina |
| Rok vydání: | 2020 |
| Předmět: | |
| Zdroj: | BioTechniques, Vol 68, Iss 5, Pp 240-244 (2020) |
| Druh dokumentu: | article |
| ISSN: | 1940-9818 0736-6205 |
| DOI: | 10.2144/btn-2019-0172 |
| Popis: | Conventional genomic DNA (gDNA) extraction methods can take hours to complete, may require fume hoods and represent the most time-consuming step in many gDNA-based molecular assays. We systematically optimized a bead bashing-based (BBB) approach for rapid gDNA extraction without the need for a fume hood. Human tissue specimens (n = 34) subjected to the 12-min BBB method yielded 0.40 ± 0.17 (mean ± SD) μg of gDNA per milligram of tissue, sufficient for many downstream applications, and 3- and 6-min extensions resulted in an additional 0.43 ± 0.23 μg and 0.48 ± 0.43 μg per milligram of tissue, respectively. The BBB method provides a simple and rapid method for gDNA extraction from mammalian tissue that is applicable to time-sensitive clinical applications. |
| Databáze: | Directory of Open Access Journals |
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