Prevention of di (2-ethylhexyl) Phthalate-induced Testicular Disturbance in Mice by Co-administration of L-carnitine
| Autor: | Zohre Zare, Moslem Mohammadi, Hossein Eimani, Majid Malekzadeh Shafaroudi |
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| Jazyk: | angličtina |
| Rok vydání: | 2011 |
| Předmět: | |
| Zdroj: | International Journal of Fertility and Sterility, Vol 5, Iss 3, Pp 186-192 (2011) |
| Druh dokumentu: | article |
| ISSN: | 2008-076X 2008-0778 |
| Popis: | Background di (2-ethylhexyl) phthalate (DEHP) is widely used in the plastic industry and can induce reproductive toxicity. On the other hand, L-carnitine (LC) plays a crucial role in sperm metabolism and maturation. This study evaluates the effect of LC on body and testis weight, testis tissue, count, motility, viability, morphology, and chromatin quality of epididymal sperm, testicular spermatid number (TSN) per gram testis and daily sperm production (DSP) in LC-treated mice. Materials and Methods In this experimental study, adult male NMRI mice (mean age: 4 weeks) were given doses of DEHP and LC by gavaging for 2 weeks. All samples were assessed according to World Health Organization (WHO) criteria. Sperm morphology was assessed using Papanicolaou staining and sperm chromatin quality by aniline-blue staining. The left testes were fixed in Bouinś solution for histological examination and the end slices were stained with hematoxylin and eosin (H&E). The right testes were homogenized, and then TSN and DSP were calculated with an improved Neubauer haemocytometer and respective frames. Paired t-test, ANOVA, and Kruskal-Wallis tests were utilized for data analysis. Results Co-administration of DEHP and LC not only prevented significant gains in testicular weight, but also maintained the sperm’s normal morphology and chromatin quality (p < 0.05). In addition, LC recovered histological changes, TSN, DSP, and sperm count. Conclusion These results demonstrated that oral administration of LC partially or generally protects spermatogenesis from DEHP-toxicity in mice. |
| Databáze: | Directory of Open Access Journals |
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