Population Diversity of n–Alkanes in the Needle Cuticular Wax of Relicts Pinus heldreichii and P. peuce from the Scardo-Pindic Mountains

Autor: Biljana M. Nikolić, Iris Đorđević, Marina Todosijević, Zorica Mitić, Srdjan Bojović, Petar D. Marin
Jazyk: angličtina
Rok vydání: 2020
Předmět:
Zdroj: Macedonian Journal of Chemistry and Chemical Engineering, Vol 39, Iss 1, Pp 41-48 (2020)
Druh dokumentu: article
ISSN: 1857-5552
1857-5625
DOI: 10.20450/mjcce.2020.1951
Popis: The variability of n-alkanes in the needle cuticular wax of Pinus heldreichii and P. peuce in two natural populations from the Scardo-Pindic mountains was investigated for the first time. Gas chromatography (GC) and gas chromatography/mass spectrometry (GC–MS) analyses of two-year-old needles were performed using an Agilent 7890A GC equipped with an inert 5975C XL EI/CI mass spectrometer detector (MSD) and a flame ionization detector (FID) connected by a capillary flow technology 2-way splitter with make-up. An HP-5MS capillary column was used. n-Alkanes ranged from C19 to C33. In P. heldreichii the most abundant were C23, C25 and C27 (16.3, 15.6 and 12.8% on average, respectively), while in P. peuce they were C29, C25, and C27 (16.5, 15.3 and 13.5% on average, resp.). Mid-chain and long-chain n-alkanes prevailed in both species. Principle component analysis (PCA) and Cluster analyses of five and six n-alkanes, respectively, showed divergence of the Scardo-Pindic populations from the Dinaric ones.
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