A Replicase of Potato virus X Acts as the Resistance-Breaking Determinant for JAX1-Mediated Resistance
| Autor: | Kyoko Sugawara, Takuya Shiraishi, Tetsuya Yoshida, Naoko Fujita, Osamu Netsu, Yasuyuki Yamaji, Shigetou Namba |
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| Jazyk: | angličtina |
| Rok vydání: | 2013 |
| Předmět: | |
| Zdroj: | Molecular Plant-Microbe Interactions, Vol 26, Iss 9, Pp 1106-1112 (2013) |
| Druh dokumentu: | article |
| ISSN: | 1943-7706 0894-0282 |
| DOI: | 10.1094/MPMI-04-13-0094-R |
| Popis: | Lectin-mediated resistance (LMR) has been suggested to comprise an uncharacterized branch of antiviral plant innate immunity. To unveil the feature of resistance conferred by jacalin-type lectin required for potexvirus resistance 1 (JAX1), a recently isolated LMR gene against potexviruses, we analyzed the resistance-breaking variants to find the viral component involved in resistance. We employed grafting-mediated inoculation, a high-pressure virus inoculation method, to obtain Potato virus X (PVX) variants that can overcome JAX1-mediated resistance. Whole-genome sequencing of the variants suggested that a single amino acid in the methyl transferase domain of the replicase encoded by PVX is responsible for this resistance-breaking property. Reintroduction of the amino-acid substitution to avirulent wild-type PVX was sufficient to overcome the JAX1-mediated resistance. These results suggest that viral replicase is involved in JAX1-mediated resistance. The residue that determines the resistance-breaking properties was highly conserved among potexviruses, suggesting a general role of the residue in potexvirus–JAX1 interactions. |
| Databáze: | Directory of Open Access Journals |
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