Establishing some Correlations between Certain Morphometric Parameters and Embryo Quality

Autor: Nicolae Păcală, Ioan Bencsik, Dorel Dronca, Gabi Dumitrescu, Ada Cean, Alexandra Ivan, Oana Ciobotaru
Jazyk: angličtina
Rok vydání: 2023
Předmět:
Zdroj: Scientific Papers Animal Science and Biotechnologies, Vol 44, Iss 1, Pp 442-442 (2023)
Druh dokumentu: article
ISSN: 1841-9364
2344-4576
Popis: The aim of this paper was to establish some correlations between certain morphometric parameters and embryo quality. The morphometric parameters taken into consideration were: zona pellucida thickness, outer and inner diameter, and outer and inner perimeter. For experiments we used embryos recovered at 24 hours from mouse females superovulated with gonadotrope hormones (eCG and hCG). The embryos recovered were cultivated in KSOM media, supplemented with amino acids, and during the in vitro cultivation they were measured at different time intervals for establishing morphometric parameters. The data obtained were statistically analyzed using Minitab 15, using Fitted Line Plot regression that allows testing of the linear and polynomial regression of one variable. After statistical analyze of the data we found that the thickness of the zona pellucida can constitute a morphometric parameter that can be used as an indicator of subsequent development of the 2 cell embryos to morula and blastocyst stage respectively. The other morphometric parameters studied (outer and inner diameter, and outer and inner perimeter) cannot be used as indicators of the embryo development.
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