| Popis: |
Pseudomonas aeruginosa, resistant to all broad-spectrum antibiotics, is on the World Health Organization's list of first-priority critical pathogens. Using reserve antibiotics, such as colistin, highlights the imperative for safer, more effective alternatives to combat these superbugs.Medical-grade honey, known for its antibacterial properties and efficacy in wound healing, emerges as a promising candidate.This study analyzed eleven honey samples, diverse in geographical, botanical, and entomological origins, for their antipseudomonal activity using the agar well-diffusion method. Zones of inhibition growth were measured in mm. The function switch was used to approximate the zones of inhibition values for antibacterial activity expressed as minimal inhibitory concentrations MIC99, MIC90, and MIC80, the lowest concentration, killing 99 %, 90 %, and 80 % of organisms, respectively. The authenticity and quality of honey samples complied with the European Commission, Codex Alimentarius, and Serbia Rulebook standards for honey. Melissopalynological analysis ensured the botanical authenticity of tested honey samples. A unique aspect of this research is that it included a honey sample from Serbia's Zlatar mountain region, harvested from Apis mellifera carnica bee colonies. The study's findings reveal that wild-type and multidrug-resistant Pseudomonas clinical isolates were susceptible to the tested honey samples. Honeydew honey from Zlatar showed the highest antibacterial activity on both tested bacteria. Honeydew and buckwheat honey from Zlatar yielded larger inhibition zones than manuka honey. Artificial honey showed no antibacterial effect.In conclusion, this research suggests the potential of honey, particularly from Zlatar, as an alternative therapy for treating infected wounds, warranting further investigation into its antimicrobial efficacy. |
